Subtract Reference Method

On the opened page, the initial tab on the top right is Subtract Reference. Click the Browse button to search for a dataset in the HDF5 file with the .img extension to use as the background image to subtract. The size of this image must match the size of the diffraction images in the 4D-STEM dataset.

Check the Apply Background Subtraction checkbox to display the result of the background subtraction in the center of the page. Once completed, click the red Start Background Subtraction button at the bottom of the page to open a dialog where you can set the output path for the resulting dataset.