Microscope Parameters

We can modify the metadata sequentially. After completing the first page (General) — and it’s okay to leave some metadata fields blank — click the Next→ button to move to the second page, which is the Microscope page. Here, you can enter information about the microscope, such as renaming it to Thermofisher FEI Titan G2 60-300.

Next, several key parameters are available for editing, including Beam Current, Accelerate Voltage, Camera Length, Convergence Angle (α), Dwell Time, and Step Size. These parameters are crucial for determining the quantitative analysis results of the dataset, so they must be modified with caution. Some parameters, like Camera Length and Convergence Angle, often require calibration using standard samples.

Edit Convergence Angle

Here, we edit the Convergence Angle. For the test dataset gold_nanoparticle_06, the convergence angle is 22.5 mrad, so in the Value field, we enter it using scientific notation as 2.25 x 10^-2 rad. Then click the OK button, and in the confirmation dialog that follows, click OK again.